In Vitro - Data Entry |
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USTUR health physics database |
 Index |
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| Select the medium and normalization type before entering any other data. |
Data Entry
The 'Medium' and the normalization 'Type' should be entered first, as automatic calculations and data entry options are dependant upon these fields. Certain normalization types are preferable to others. The normalization types accommodated by the USTUR database, listed from most to the least desirable normalization type, are:
24-hr = 24-hour sample
Sim24 = Simulated 24-hour sample
Pooled = Effective sample period of several pooled samples is used to calculate excretion rate.
Timed = Sample period (Time) is used to calculate excretion rate.
Volume/Mass = Sample Volume or Mass is used to calculate excretion rate.
Conc = Sample Concentration is used to calculate excretion rate.
Creatinine = Creatinine is used to calculate excretion rate.
n.a. = Hard file contains insufficient data to calculate excretion rate.
The procedures for choosing a normalization type and entering the sample period, collection start time, collection end time, and sample volume are depicted as a 'decision tree' in the below figure.
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Decision tree for choosing the normalization type for urine and fecal samples. |
Once the normalization type has been selected, other data can be entered according to the following guidelines:
24-hour sample (24-hr)
Every void during 24 consecutive hours is collected and combined into a single 24-hr sample.
- Data Entry:
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Medium – Select Urine or Feces from the dropdown menu.
Type – 24-hr.
Collection Start Time – Enter the collection start time.
Collection End Time – Enter the collection end time. (The difference between start and end times must be exactly 1.0 day).
Effective Sample Period – 1.0 day (automatically entered).
Override – Checkbox is unchecked and disabled.
Volume/Mass – Enter volume (urine or blood) or wet mass (feces) if known. Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed. Calculations are based upon the analyzed value.
Volume/Mass Unit – Select the unit used to report the volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Simulated 24-hour sample (Sim24)
Individual sample periods may or may not sum to 24-hours, but they are designed to simulate the activity that would be excreted during a single 24-hour period.
- Examples:
-
Two separate 12-hour urine samples are collected and combined into one composite sample (sample periods that add to 24-hours may indicate a Sim24).
-
Medium – Select Urine or Feces from the dropdown menu.
Type – Sim24.
Collection Start Time – Enter the first sample's collection start time (start time is not used in dose assessments, but is included for data completeness).
Collection End Time – Enter the last sample's collection end time (if hard file contains only one time, but does not indicate whether it is a start or an end time, interpret it as a collection end time). The difference between start and end times will vary from 1.0 day.
Effective Sample Period – 1.0 day (automatically entered).
Override – Checkbox is unchecked and disabled.
Volume/Mass – Enter the volume (urine or blood) or wet mass (feces) if known. Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed. Calculations are based upon the analyzed value.
Volume/Mass Unit – Select the unit used to report the volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Pooled sample (Pooled)
Effective sample period of several combined (Pooled) samples is used to calculate excretion rate. Essentially this is a simulated timed sample for a time period other than 24-hours.
- Example:
- Three separate 12-hour urine samples are collected and combined into a single composite sample (36-hr effective sample period).
-
Medium – Select Urine or Feces from the dropdown menu.
Type – Pooled.
Collection Start Time – Enter the first sample’s collection start time (start time is not used in dose assessments, but is included for data completeness).
Collection End Time – Enter the last sample’s collection end time (if hard file contains only one time, but does not indicate whether it is a start or an end time, interpret it as a collection end time);
Effective Sample Period – Manually enter the simulated sample period;.
Override – Checkbox is unchecked and disabled.
Volume/Mass – Enter the volume (urine or blood) or wet mass (feces) if known. Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed. Calculations are based upon the analyzed value.
Volume/Mass Unit – Select the unit used to report the volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Timed Sample (Timed)
Sample period is used to calculate excretion rate. If the sample period is known, the average excretion rate over the collection period can be calculated.
- Example:
- Several samples are collected over the course of 10 hours and combined into one composite sample.
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Medium – Select Urine or Feces from the dropdown menu.
Type – Timed.
Collection Start Time – Enter the collection start time.
Collection End Time – Enter the collection end time.
Effective Sample Period – Access automatically calculates the sample period from the collection start and end times.
Override – Checkbox is unchecked and disabled.
Volume/Mass – Enter volume (urine or blood) or wet mass (feces) if known. Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed. Calculations are based upon the analyzed value.
Volume/Mass Unit – Select the unit used to report the volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Volume/Mass Sample (Volume/Mass)
Volume is used to calculate excretion rate. If the sample volume (urine or blood) or mass (feces) is known, the average excretion rate over the collection period can be calculated using the daily excretion rate of reference man.
- Example:
- A single urine sample was collected. The volume is known, but the sample period is not known.
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Medium – Select Urine, Feces, or Blood from the dropdown menu.
Type – Volume.
Collection Start Time – Enter the collection start time if known (if both start time and end time are known, data type should be ‘Timed’ not ‘Volume’).
Collection End Time – Enter the collection end time if known (if hard file contains only one time, but does not indicate whether it is a start or an end time, interpret it as a collection end time).
Effective Sample Period – Leave blank (if sample period is known, data type should be ‘Timed’ not ‘Volume’).
Override – Checkbox is unchecked and disabled.
Volume/Mass – Enter the volume (urine or blood) or wet mass (feces). Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed. Calculations are based upon the analyzed value.
Volume/Mass Unit – Select the unit used to report the collected volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Concentration Sample (Conc)
Concentration used to calculate excretion rate. If the concentration of a urine or fecal sample is known, the average excretion rate over the collection period can be calculated using the daily excretion rate of reference man.
- Example:
-
Only the sample's concentration is reported.
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Medium – Select Urine, Feces, or Blood from the dropdown menu.
Type – Conc.
Collection Start Time – Enter the collection start time if known.
Collection End Time – Enter the collection end time if known (if hard file contains only one time, but does not indicate whether it is a start or an end time, interpret it as a collection end time) – if volume or mass and both start time and end time are known, data type should be ‘Timed’ not ‘Conc’.
Effective Sample Period – Automatically calculated as End Time - Start Time. If manual data entry is preferred, click to override the automatic sample period calculation (if volume or mass and sample period are both known, data type should be ‘Timed’ not ‘Conc’).
Override –Checkbox is unchecked and disabled.
Volume/Mass – Leave blank. If the analyzed volume or mass is known, data type should be ‘Volume’ not ‘Conc’ (Collection volume or mass data may be known if the sample volume differs from the analyzed volume).
Volume/Mass Unit – Select the unit used to report the collected volume or mass in the hard file (Access will automatically calculate volume in mL or mass in g).
Creatinine Sample (Creatinine)
Creatinine level is used to calculate urinary excretion rate. If the level of creatinine in a urine sample is known, the average urinary excretion rate over the collection period can be calculated using the daily creatinine excretion rate of reference man (1.7 g/day - ICRP 89).
- Example:
-
The sample activity and the amount of creatinine in the sample are known.
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Medium – Select Urine from the dropdown menu.
Type – Creatinine.
Collection Start Time – Enter the collection start time if known.
Collection End Time – Enter the collection end time if known (if hard file contains only one time, but does not indicate whether it is a start or an end time, interpret it as a collection end time) – if both start time and end time are known, data type should be ‘Timed’ not ‘Creatinine’.
Effective Sample Period – Automatically calculated as End Time - Start Time.
Override – Uncheck to disable the sample period override and revert to an automatic sample period calculation.
Volume/Mass – Enter the volume (urine). Collection Volume/Mass refers to the entire sample. Analyzed refers to the Aliquot that was analyzed.
Volume/Mass Unit – Select the unit used to report the collected volume in the hard file (Access will automatically calculate volume in mL).
n.a. Sample (n.a.)
The hard file does not contain enough time, volume, or concentration data to calculate the average excretion rate. The n.a. sample type also applies to nasal and oral smears since they are not used to calculate an excretion rate.
- Example:
- A single spot sample is collected. The collection time is known, but not the sample volume.
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Medium – Select Urine, Feces, Blood, Nasal Smear, or Oral Smear from the dropdown menu.
Type – n.a.
Collection Start Time – Enter the collection start time if known (not applicable for nasal and oral smears).
Collection End Time – Enter the collection end time if known (if both start time and end time are known, data type should be ‘Timed’ or ‘Conc’ not ‘Limited’). Enter nasal and oral smear times as an end time.
Effective Sample Period – Leave blank (if sample period is known, data type should be ‘Timed’ or ‘Conc’ not ‘n.a.).
Volume/Mass – Leave blank (if Collection Volume or Mass is known, data type should be ‘Volume’ not ‘n.a.).
Volume/Mass Unit – Not applicable.
Note: Beware container volumes that are recorded as sample volumes (i.e. a 700 mL sample is submitted in a 1 L bottle and recorded as a 1 L sample). This will not impact 'Timed' samples, but it will lead to invalid 'Volume' and 'Conc' excretion rate calculations.
Other Data Fields
These fields to do not depend upon the sample type.
- Data Entry:
-
Chelation – Yes (checked) = Urine, Feces or Blood sample was taken within 14 days of chelation treatment. The chelation checkbox is automatically entered based upon chelation entries in the 'Treatment' tab.
Contaminant(s) – Select the contaminant (radionuclide or type of radiation) or mixture from the drop down list.
Reported Net Activity Value – Enter the net activity as it appears in the hard file (do not perform unit conversions).
Reported Net Activity Uncert – Enter the uncertainty on net activity as it appears in the hard file (no unit conversions).
Reported Net Activity MDA – Enter the net activity’s minimum detectable activity as it appears in the hard file (no unit conversions).
Reported Net Activity DL – Enter the net activity’s Decision Level as it appears in the hard file (no unit conversions).
Reported Net Activity Unit – Select the unit used to report the activity, uncertainty, MDA, and DL in the hard file.
Reported <MDA – Check this box if the reported activity levels were less than the minimum detectable activity. Background activities are to be entered as having 0 net activity and the <MDA checkbox is to be checked. If one record reports a sample activity at background levels and another reports an activity that is greater than zero, enter the numerical value as the reported net activity, check the 'Reported <MDA' checkbox, and comment on this observation in the 'Comments' field.
Creatinine – Enter the grams of creatinine in the entire urine sample. If this is scaled up from an aliquot volume, indicate the mass of creatinine measured in the aliquot in the comments.
Count Start Time – Enter the count start time.
Count End Time – Enter the count end time.
Count Length – Enter the length of count.
Comments – Enter any additional pertinent information.
Laboratory – Indicate the laboratory where sample was analyzed, if known.
Source – Enter the source of in vitro data as: document date – document name (i.e. 5/22/79 - internal dosimetry memorandum from site health physicist). Do not include specific personnel or registrant names.
This page was last updated on May 22, 2008. usturwebmaster@tricity.wsu.edu
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